DNaseI treatment is a prerequisite for the amplification of cDNA from episomal-based genes.
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The mRNAexpression study on small amount of Theileria annulata lymph node biopsy sample using SMART-cDNA technology
BACKGROUND: A major issue in many gene expressionstudies utilizing small amount of biological materials is thelimited quantity of RNApurified from clinical samples, which isoften used for RT-PCR or standard Northern blot analysis.OBJECTIVES: The SMART cDNA synthesis method and subsequentSMART-cDNA-PCR technique was used to analyse 3genes in macroschizonts of Theileria annulata in small lymphnod...
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Extended Abstract Introduction and Objective: Polyploidy is one of the main factors in plant adaptation that can increase secondary metabolites production in plants. Salvia officinalis L. is a perennial plant from the Lamiaceae family with a long history of use in the medicinal industry. Tanshinones are crucial active compounds biosynthesized in Salvia. This study was aimed to analyze the expr...
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Senescence is a genetically regulated oxidative process that involves a general degradation of cellular structures and enzymes and the mobilization of the products of degradation to other parts of the plant. The cDNA-AFLP (cDNA-Amplified Fragment Length Polymorphism) analysis has been used under stringent PCR conditions afforded by ligation of adapters to restriction fragments, and the use of s...
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Abstract Background: Factor VII, is a coagulant protease it begins the proteolytic cascade reactions and produces thrombin. The use of recombinant human factor VII, (rhFVII) is effective for the treatment of patients with hemophilia A or B. It is a target for gene therapy. This study was done to clone factor VII from HepG2 cell line. Methods: RNA was extracted from the hepatoma, (HepG2), ...
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A multiplex PCR-based assay was developed for detection of Salmonella typhi and identification of other salmonella serotypes. Three primer-sets were selected from different genomic sequences, malo2-F/malo2-Ra primers from invasion gene, Parat-s/Parat-as as well as tyv-s/tyv-as primers from O-antigen gene cluster of the genus Salmonella. This method differentiated Salmonella spp., based on size ...
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عنوان ژورنال:
- BioTechniques
دوره 20 4 شماره
صفحات -
تاریخ انتشار 1996